Jean Peccoud has experienced first hand the challenges of managing research programs that combine experimental and computational elements. He is passionate about increasing the productivity of his own research and hopes to help other groups by blogging about his experience. Jean is also the founder of GenoFAB.
This protocol describes the entire life cycle of a mammalian cell culture. It starts with seeding the flask and finishes with harvesting, counting, and the preparation of a normalized cell suspension. It is written for HEK297 but can be adapted for other cells.
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